Tabriz University of Medical Sciences Pharmaceutical Sciences 1735-403X 19 4 2014 03 01 Advanced Glycation End Product Inhibitory Effect of Quercetin-3-O-Rutinoside, Isolated from Teucrium polium 125 134 EN Mohammad Ali Esmaeili Journal Article 2014 02 25 Background: Several mechanism including autoxidation of glucose, protein glycation and advanced glycation end products (AGEs) formation may to be involved in hyperglycemia mediated oxidative stress. Herein, in this study, the effects of rutin with high antioxidant activity isolated from Teucrium polium on structural changes of human serum albumin (HSA) were performed. Methods: The major compounds of T. polium methanol extract were isolated and their radical scavenging properties were established through various approaches including 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radicals scavenging, and reducing power. The structural changes of HSA with glucose, in the presence of rutin were studied with far-UV CD spectroscopy. Results: Based on our results, rutin, a flavonol glycoside isolated from T. polium exhibited high antioxidant activity compared to the other isolated compounds from T. polium. Rutin inhibited high glucose induced oxidative damages to protein by decreasing protein carbonyl formation (PCO) and preventing thiols group from oxidation. In addition, evaluating the structural changes of HSA by glucose in the presence of rutin showed that rutin may be able to increase the helicity of the protein and prevent helix decrement in the secondary structure of HSA. Conclusion: We found that rutin with high antioxidant activity effectively inhibited the generation of amadori products and exhibited good inhibitory effects on the formations of α-dicarbonyl compounds and AGEs.